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| Synonym: | 2,2′-(Bisacrylamino)diethyl disulfide, BAC, Bis(2-acrylamidoethyl) disulfide |
| CAS Number: | 60984-57-8 |
| Linear Formula: | C10H16N2O2S2 |
| Molecular Weight: | 260.38 |
| EC Number: | 262-546-8 |
| MDL number: | MFCD00036225 |
Description
| Application | BAC is used as a reversible cross-linker for polyacrylamide gels. The disulfide linkage can be broken with a suitable reducing agent. A 12:1 ratio of acrylamide:BAC is recommended for DNA purification. A one gram slice of a 4% gel will dissolve in 5 mL of 100 mM DTT, TBE solution (pH 8.3) in less than 30 min. The pH of 8.3 is optimum for rapid dissolution of the gel. |
Properties
| assay | ~98% (HPLC) |
| solubility | acetic acid: 50 mg/mL |
| storage temp. | −20°C |
Safety
References
| reference | Hansen, H.J., Electrophoresis of ribonucleic acid on a polyacrylamide gel which contains disulfide cross-linkages. Anal. Biochem. 76, 37-44, (1976) |
| | Hansen, H.J., Chemical and electrophoretic properties of solubilizable disulfide gels. Anal. Biochem. 105, 192-201, (1980) |
